Evidence for mediated protein uptake by amphibian oocyte nuclei.
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چکیده
منابع مشابه
Evidence for mediated protein uptake by amphibian oocyte nuclei
The objective of this investigation was to determine whether there is mediated transport of endogenous proteins across the nuclear envelope. For this purpose, we studied the nuclear uptake of a 148,000-dalton Rana oocyte polypeptide (RN1) and compared its actual uptake rate with the rate that would be expected if RN1 crossed the envelope by simple diffusion through the nuclear pores. Nuclear up...
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E. Mathilda SJOBERG,*§ Anna BLOM,* Bengt S. LARSSON,t James ALSTON-SMITH,* I Mats SJOQUIST,T and Erik FRIES*¶ *Department of Medical and Physiological Chemistry, University of Uppsala, Biomedical Centre, Box 575, S-751 23 Uppsala, Sweden, tDepartment of Pharmaceutical Biosciences, Division of Toxicology, University of Uppsala, Biomedical Centre, Box 594, S-751 24 Uppsala, Sweden, and tDepartmen...
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An in vitro assay for nucleocytoplasmic transport was established in which signal-dependent protein import is reproduced faithfully by isolated purified nuclei. The assay permits the precise quantification of import kinetics and the discrimination between translocation through the nuclear envelope and intranuclear transport. Nuclei were manually isolated from Xenopus oocytes and after manual pu...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1983
ISSN: 0021-9525,1540-8140
DOI: 10.1083/jcb.96.5.1486